Fig. 5.

NPD1 protects against caspase cleavage induced by TNF-α/H₂O₂. ARPE-19 cells were serum-starved for 1 h before a 6- to 8-h treatment with TNF-α (10 ng/ml)/H₂O₂ (either 400 or 800 μM as indicated) in the presence or absence of NPD1 (50 nM). (A) Caspase-3 cleavage was detected by Western blot analysis by using poly(ADP-ribose) polymerase (Santa Cruz Biotechnology) as antibody. (B) Cleavage of caspase-3 using cells stably transfected with a lentivirus construct containing the Asp-Glu-Val caspase-3 cleavage sequence. Red bars represent coincubations with 50 nM NPD1. The values are the average of triplicate samples in three independent experiments.