RP-HPLC-HR-ESMS chromatograms of CRM 7405-a (Hijiki) after extraction
with DCM/MeOH (2 + 1): (a) the crude extract, (b) extract after silica
cleanup, and (c) extracted m/z for
each arsenolipid species after silica cleanup; all as thio analogues.
ZORBAX Eclipse XDB-C8 (4.6 × 150 mm, 5 μm); mobile phase,
water/ethanol gradient (70–90% EtOH, incl. 0.1% formic acid);
flow rate, 1 mL min–1; column temperature, 30 °C;
injection volume, 5 μL. The following ions (m/z), as their thio derivatives, were extracted:
AsHC332–349.1914; AsHC360–377.2228; AsPL958–975.4993;
AsPL986–1003.5327; AsPL1014–1031.5647; AsPL1042–1059.5949;
AsPL1070–1087.6228.