Figure 3.

Gap19 inhibits hemichannel activity in astrocytes studied in acute hippocampal slices. (A) Representative images of Etd⁺ uptake (red) in astrocytes (green) in hippocampal slices from GFAP-eGFP transgenic mice, under control conditions (upper row) and after 10 min exposure to a Ca²⁺-free solution without (middle row) or with (lower row) 344 μM Gap19 treatment. Scale bar: 10 μm. (B) Summary graph demonstrating significant Etd⁺ uptake in astrocytes that was inhibited by Gap19 used at concentration of 344 and 688 μM. Statistical comparisons in (B) were done with the stimulus condition without Gap19 (n = 3 independent experiments; ^***p < 0.001).