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. Author manuscript; available in PMC: 2014 Oct 21.
Published in final edited form as: Methods Mol Biol. 2012;920:53–78. doi: 10.1007/978-1-61779-998-3_5
(a) 5× Herculase II reaction buffer 10 µL
(b) dNTPs (25 mM each) 0.5 µL
(c) Template DNA 10 ng
(d) Forward primer (10 µM) 1.25 µL
(e) Reverse primer (10 µM) 1.25 µL
(f) Nuclease-free water to 49.5 µL