(a) 2× binding buffer | 5 µL |
(b) 33P-labeled f298 probe | 20 fmole |
(c) Recombinant Ku | 10 fmole |
(d) Recombinant XRCC4/Ligase IV | 40 fmole |
(e) Nuclease-free water | to 10 µL |
(a) 2× binding buffer | 5 µL |
(b) 33P-labeled f298 probe | 20 fmole |
(c) Recombinant Ku | 10 fmole |
(d) Recombinant XRCC4/Ligase IV | 40 fmole |
(e) Nuclease-free water | to 10 µL |