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. Author manuscript; available in PMC: 2014 Oct 21.
Published in final edited form as: Methods Mol Biol. 2012;920:53–78. doi: 10.1007/978-1-61779-998-3_5
(a) 10× kinase buffer 2 µL
(b) f298 DNA 10 pmole
(c) γ-33P-dATP (3,000 Ci/mmole, 10 mCi/mL) 20 pmole (6 µL)
(d) Nuclease-free water to 19 µL