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. Author manuscript; available in PMC: 2014 Oct 21.
Published in final edited form as: J Neurochem. 2013 Mar 11;125(3):420–429. doi: 10.1111/jnc.12204

Fig. 2.

Fig. 2

Embryonic neural stem/progenitor cells (NSPCs) are more resistant to cytochrome c oxidase inhibition but more susceptible to glycolytic and pentose phosphate pathway inhibition than neurons. (a) Viability of cyanide-exposed (1 mM; 24 h) neurons and eNSPCs as measured using calcein AM fluorescence and compared as a percent of untreated controls. (b) Neurons and eNSPCs were treated with 10 mM 2-deoxy-D-glucose (2-DG) and 10 mM glucose, in the presence of increasing concentrations of pyruvate (0.1–10 mM) for 24 h. Viability of neurons (open circles) and eNSPCs (closed circles) as compared as a percent of controls that were not treated with 2-DG. Two-way ANOVA shows a significant difference between cell type (p < 0.0001) and concentration of pyruvate (p < 0.001). (c) Viability of neurons and eNSPCs treated with 25 mM galactose as measured using calcein AM fluorescence and compared as a percent of glucose-treated (25 mM) controls. (d) Viability of neurons and eNSPCs exposed to 500 μM 6-AN for 24 h as compared to vehicle (dimethylsulfoxide) controls. n = 3 per treatment group. *p < 0.05. ***p < 0.001. ****p < 0.0001.