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. Author manuscript; available in PMC: 2014 Oct 21.
Published in final edited form as: J Neurochem. 2013 Mar 11;125(3):420–429. doi: 10.1111/jnc.12204

Fig. 5.

Fig. 5

Deletion of hypoxia inducible factor-1alpha (Hif1a) does not alter the metabolic phenotype of embryonic neural stem/progenitor cells (NSPCs). (a) PCR of genomic DNA showing successful recombination between the loxP sites in the Hif1a gene from YFP+ nestin-CreERT2: R26R-YFP:Hif1aΔ/Δ eNSPCs (Hif1aΔ/Δ). (b–f) 24 h treatment of nestin-CreERT2:R26R-YFP:Hif1awt/wt (Hif1awt/wt) versus nestin-CreERT2: R26R-YFP:Hif1aΔ/Δ (Hif1aΔ/Δ) eNSPCs isolated from mice exposed to (b) anoxia, (c) CN (1 mM), (d) 6-AN (500 μM), (e) galactose (25 mM), or (f) 2-DG (10 mM) plus glucose (10 mM) and pyruvate (0.1–10.0 mM). Two-way ANOVA shows a significant difference between genotype (*p < 0.05) and concentration of pyruvate (**p < 0.01) for (f). n = 3 per treatment group.