Table 2.

Continuous CREB antisense infusion does not affectl-DOPA-induced changes in gene expression

Gene expression markers	Acutel-DOPA		Chronic l-DOPA
	CREB AS	Control oligo	CREB AS	Control oligo
Number of c-Fos+ cells	144  ± 31	106  ± 31	9  ± 4	8  ± 3
Number of FosB+ cells	42  ± 9	63  ± 18	140  ± 32	170  ± 62
PDyn mRNA (kBq/gm)	976  ± 89	1061  ± 71	2250  ± 325	1881  ± 541

Data were collected from animals sustaining continuous oligonucleotide infusion in the lateral caudate–putamen for 14 d.l-DOPA (8 mg/kg per injection, i.p) was administered either on day 14 (acute treatment) or on days 2–14 (chronic treatment). The three genes under investigation show a differential response to the effects of chronic versus acute l-DOPA treatment: higher levels of PDyn mRNA and FosB-like proteins are seen after chronic treatment, and higher levels of c-Fos expression are found after acute treatment. However, none of the observed changes is affected by CREB antisense.