Table 5.
Activity of antioxidant enzymes (normalized to mg of protein) and concentrations of ascorbate and phenylpropanoids in leaves of well-watered and salt-treated F. ornus grown at 45 (shade) or 100 % (sun) solar irradiance
| Parameter | Shade |
Sun |
NIVlight | NIVsalt | ||
|---|---|---|---|---|---|---|
| C | ST | C | ST | |||
| SOD (U) | 0·26 ± 0·04 | 0·30 ± 0·04 | 0·57 ± 0·03 | 0·62 ± 0·06 | +0·35 | +0·04 |
| CAT (mmol H2O2 min–1) | 10·4 ± 1·5 | 7·8 ± 0·9 | 15·3 ± 2·30 | 18·6 ± 1·24 | +0·30 | +0·00 |
| APX (mol ASA min–1) | 0·17 ± 0·02 | 0·06 ± 0·02 | 0·15 ± 0·03 | 0·25 ± 0·01 | +0·27 | −0·01 |
| POX (μmol oxidized guaiacol) | 0·24 ± 0·04 | 0·53 ± 0·06 | 0·38 ± 0·02 | 0·34 ± 0·04 | −0·03 | +0·16 |
| PPO (μmol quinones) | 0·15 ± 0·03 | 0·63 ± 0·06 | 0·14 ± 0·02 | 0·36 ± 0·04 | −0·22 | +0·55 |
| ASA (μg g–1 DW) | 48·0 ± 5·6 | 56·5 ± 4·4 | 74·7 ± 6·0 | 60·5 ± 5·8 | +0·05 | −0·02 |
| DHASA (μg g–1 DW) | 60·2 ± 3·6 | 24·3 ± 5·1 | 84·1 ± 4·3 | 98·4 ± 8·0 | +0·38 | −0·08 |
| Esculetin (μmol g–1 DW) | 45·1 ± 7·1 | 68·1 ± 5·5 | 45·9 ± 5·7 | 43·4 ± 4·1 | −0·12 | +0·12 |
| ESC (μmol g–1 DW) | 54·1 ± 3·6 | 40·6 ± 5·0 | 58·2 ± 3·7 | 60·5 ± 7·2 | +0·11 | −0·04 |
| QUE (μmol g–1 DW) | 4·3 ± 0·8 | 12·9 ± 1·8 | 8·9 ± 0·7 | 8·7 ± 1·1 | +0·01 | +0·24 |
Data are mean ± s.d. (n = 4).
Leaves sampled for measurement were fully developed (5–6 weeks old), collected at midday.
ESC, esculin (esculetin 6-O + esculetin 7-O-glucoside); QUE, quercetin 3-O-glycosides.