Table 2.

Sensitivity analysis for impact of microrate constants on Km and Vmax for the six-state model. Values in red (and underlined) denote changes that were over 1000-fold higher or lower. k_n is either k₁-k₋₁ and denotes the microrate constant that was varied. For example, compared to when all microrate constants were 1 µM⁻¹s⁻¹ or 1 s⁻¹, increasing k₁ to 1,000,000 µM⁻¹s⁻¹ decreased Km by 1,000,000-fold (i.e. 0.000001-fold change, noted in table). Meanwhile, compared to when all microrate constants were 1,000,000 µM⁻¹s⁻¹ or 1,000,000 s⁻¹, decreasing k₁ to 1 µM⁻¹s⁻¹ increased Km by 1,000,000-fold.

kn	Fold change in Km when…		Fold change in Vmax when…
	kn increased to 1,000,000 a	kn decreased to 1 b	kn increased to 1,000,000 a	kn decreased to 1 b
k1	0.000001	1000000	1	1
k2	0.75	1.50	1.50	0.000003
k3	1.50	0.000003	1.50	0.000003
k4	1.50	0.000003	1.50	0.000003
k−1	500000.5	0.50	1	1

^aSensitivity analysis involved increasing k_n from a value of 1 to a value of 1,000,000, while all other microrate constants retained a value of 1.

^bSensitivity analysis involved decreasing k_n from a value of 1,000,000 to a value of 1, while all other microrate constants retained a value of 1,000,000.