Fig. 2.

Representative yeast two-hybrid results for MADS-domain proteins from A. trichopoda. Photographs show colony growth on selective –Leu/–Trp/–His media with yeasts grown at 22 °C. For each interaction tested, yeast cells were spotted in a 10-fold serial dilution (from left to right). Proteins that were expressed as fusions with the GAL4 DNA-binding domain (vector pGBKT7) are shown horizontally; proteins expressed as GAL4 activation domain fusions (vector pGADT7) are shown vertically. Δ indicates negative controls in which empty vectors that did not contain a MADS-box gene cDNA insert were used.