Fig. 2.

Effect of the moss mutation on the expression of TFL1. (A) Expression of TFL1::GUS in wild-type (WT) and moss shoot apices in different developmental stages. Apices from plants at equivalent developmental stages were compared. In late inflorescences, GUS signal in the WT apex is restricted to the inflorescence meristem (arrowhead), while in the moss apex signal is also observed in the flowers (arrows). The SAM in the vegetative and early inflorescence apices is outlined with a pink line. (B) mRNA levels of TFL1 and GUS in the inflorescence apices of WT (Ler pBTG6) and moss plants, determined by RT-qPCR. Samples were from main inflorescence apices from 40-day-old plants (= late inflorescence). The mRNA level of the UBIQUITIN 10 gene was used as reference. Values represent the means of three technical replicates ± standard error. Scale bars: 300 μm in vegetative and early inflorescence apices; 2 mm in late inflorescence apices.