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. 2014 Oct 21;12(10):e1001974. doi: 10.1371/journal.pbio.1001974

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© 2014 Oh et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) A confocal image of pdf-Gal4/UAS-EGFP fly brain stained with anti-MIP (green) and anti-EGFP (magenta) antibodies. The brain is oriented with dorsal up. (B) A high magnification confocal image of the optic lobe of pdf-Gal4/UAS-DenMark fly brain stained with anti-MIP (green) and anti-RFP (magenta) antibodies. Anti-RFP labels a dendrite marker, DenMark, which visualizes the dendritic field of pdf neurons. Asterisks indicate somata of MIP-LMIo neurons. Sections perpendicular to the dotted yellow lines are shown separately in (B′) and (B″). (B′) shows MIP-immunoreactive processes (yellow arrows) innervate the dendritic field of pdf neurons (yellow arrowheads). (B″) shows some MIP-labeling (arrows) occurs near the dendritic field of pdf neurons (arrowheads), indicating that MIP can also function as a paracrine factor. White arrows labeled with d, a, m indicate dorsal, anterior, and medial orientations, respectively. Scale bars, 50 µm.