Figure 1. Percent viability of T cells labeled with various concentrations of DiR measured using ATP based luminescent assay.

Bryostatin/Ionomycin (B/I) activated 4T1-cells grown in IL-2 were labeled on day 6 of their ex vivo expansion, which is day-0 of labeling. Viability of T-cells on 1, 4 and 7 days post-labeling were compared with unlabeled cells on respective days and expressed as percent viability. T-test was used to compare the significant differences (p<0.05) in the cell viability between day 1 versus day 7 (*) and day 4 versus day 7 (#). Average ± SD from three different assays are shown in the graph.