Table 1.

PCR and sequencing primers

Primer	Target gene	Position	Use	Sequence (5'-3')	Reference
23Scom a	23S rRNA	6-26b	PCR/Seq	AGTTAATAAGGGCGCACGGTG	This work
23Sg1 c	23S rRNA gene1	1577119-157741d	PCR/Seq	AAGACGTATTGAAGCTTACTCTA	This work
23Sg2 c	23S rRNA gene2	22879-22857 d	PCR/Seq	AGCTACTTCCCGAACTGATGCAC	This work
23Sg3 c	23S rRNA gene3	29128-29106 d	PCR/Seq	GATAACCGTCTTCTTTCCCCTTT	This work
23Sg4 c	23S rRNA gene4	85192-85170 d	PCR/Seq	CATATTTCTAACACGGGCAGTAG	This work
23Sg5 c	23S rRNA gene5	270607-270582 d	PCR/Seq	CACTGCCAAGCTATCTAAACGTA	This work
23Sg6 c	23S rRNA gene6	1330037-1330059d	PCR/Seq	CAATTGAATAGCCTGCACGTTCG	This work
MS2 e	23S rRNA	550-573b	Sequencing	CGTGTGCCTACAACAAGTTCGAGC	This work
MS3 e	23S rRNA	573-550b	Sequencing	GCTCGAACTTGTTGTAGGCACACG	This work
MS4 e	23S rRNA	1016-997b	Sequencing	TTTCGACTACGGATCTTAGC	[11]
MS5 e	23S rRNA	867-885b	Sequencing	CTGTTTGGGTGAGGGGTCC	[11]
MS6 e	23S rRNA	1424-1446b	Sequencing	GGACGCAGTAGGCTAACTAAACC	This work
MS7 e	23S rRNA	1446-1424b	Sequencing	GCTTTAGTTAGCCTACTGCGTCC	This work
MS8 e	23S rRNA	2021-2040b	Sequencing	ACCTGCATCTTCACAGGTAC	[11]
MS9 e	23S rRNA	1902-1921b	Sequencing	CGGCGGCCGTAACTATAACG	[11]
MS10 e	23S rRNA	2415-2437b	Sequencing	GCCATCGCTCAACGGATAAAAGC	This work
MS11 e	23S rRNA	2437-2415b	Sequencing	GCTTTTATCCGTTGAGCGATGGC	This work
MS12 e	23S rRNA	2904-2880b	Sequencing	TTGGATAAGTCCTCGAGCTATTAG	[11]
L4fu	L4	64481-64498c	PCR/Seq	GCAAACACAGCTCCTAAG	This work
L4rd	L4	65751-65734c	PCR/Seq	CTGTGTAACGACCAACGC	This work
L4f156	L4	65074-65091c	Sequencing	AACCGTTCAGCAGTATCA	This work
L4r444	L4	65307-65290c	Sequencing	TGAAAGGCCTTCTACAGC	This work
L22-1	L22	67079-67098c	PCR/Seq	GCAGACGACAAGAAAACACG	[11]
L22-2	L22	67500-67481c	PCR/Seq	ATTGGATGTACTTTTTGACC	[11]

^a A PCR primer common to all 23S rRNA genes ^b Position in the 23S rRNA molecule (E. coli accession number J01695, numbering). ^c rRNA operon spesific PCR primers, artificial numbering of the 23S rRNA genes of the S. pyogenes ^d Position in the S. pyogenes genome (accession number AE004092). ^e23S rRNA gene sequencing primers