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NIH3T3 cells were co-transfected with the Per2 promoter reporter (400 ng) and the indicated expression plasmids (300 ng) for HIF1α/ARNT or empty vector pcDNA3 (600 ng) as a control. Plates were read on the ArrayScan XTI (Thermo Scientific) for cell count indicated time after dexamethasone treatment. Numbers of viable cells were not affected by HIF1α/ARNT at all time points (mean ± SEM, n = 6, Student's t-test).
