Table 2. Prediction of Arabidopsis candidate proteins for S-nitrosylation using the GPS-SNO 1.0 software.

	Arabidopsis proteome	Candidate proteins for S-nitrosylation	The highest 10% high-confident predicted candidates
Total number of proteins	27,416	16,610 (60%)	3,005 (18%)
Total number of Cys-NO	207,473	31,907 (15%)	3,190 (10%)

Arabidopsis amino acid sequences were extracted from TAIR 10 database (www.arabidopsis.org) and analysed by GPS-SNO 1.0 software using medium threshold condition. The 10% of predicted sites with the highest prediction confidence were determined by ranking the prediction results according to the raw score divided by the threshold (Cutoff) for a particular cluster.