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. 2014 Oct 21;9(10):e110422. doi: 10.1371/journal.pone.0110422

Figure 2. Southern and Northern blot analyses.

Figure 2

A. Ten µg/lane of EcoRI-digested genomic DNA from Sf9 cells was hybridized with a mono-exonic (exon 3, {E3l}) digoxigenin-labeled Fut8 probe prepared by PCR using the Forint10 and B24 primers (Table S1), as described in Materials and Methods. B. One µg/lane of total RNA was analyzed by Northern blotting. Hybridization was performed with a fut8 anti-sense RNA probe (pos. 825 to pos. 1686 on the cDNA, Figure 1). Molecular weight markers in bp are indicated on the left of each panel.