Table 1. Determination of the optimal sequence for initiation of translation in Sf9 cells.
Sequences analyzed n = 168 | Position upstream of the start codon* | ||||||||||
-10 | -9 | -8 | -7 | -6 | -5 | -4 | -3 | -2 | -1 | AUG | |
A | 43 | 36 | 27 | 35 | 27 | 22 | 39 | 88 | 64 | 57 | |
U | 26 | 34 | 29 | 27 | 31 | 41 | 11 | 0 | 13 | 6 | |
G | 14 | 17 | 14 | 7 | 28 | 12 | 7 | 10 | 3 | 11 | |
C | 17 | 13 | 30 | 31 | 14 | 25 | 43 | 2 | 20 | 26 | |
Consensus sequence | A | A/U | A/U/C | A/U/C | A/U/G | U | A/C | A | A | A | AUG |
168 genes expressed in Sf9 cells [40] were included in this analysis.
*Nucleotide flanking Sf9 start codons, in percentage.