Table 3. Determination of α1–6-fucosyltransferase activity.
| Sample | α1,6-fucosyltransferase activity |
| 2D production | 1,229+/−80 |
| 3D production | 2,554+/−35 |
| Negative control | 62+/−1 |
Recombinant FLAG-tagged Sf9 FUT8 was produced using the baculovirus-Sf9 cell system and purified using anti-FLAG M2 affinity gel. The fucosyltransferase activity of the recombinant protein was tested with GDP-[14C]-L-fucose, as substrate, after two days (2D) and three days (3D) of production. The transfer of [14C]-fucose is expressed in cpm.