Figure 3.

PLC assays in PLCδ4 overexpressing cells and expression of GFP-PH domain/PLCδ4 fusion protein in MCF-7 cells. Assay of enzymatic activities of PLCδ4 overexpressing clones in MCF-7 cell extracts were carried out using PIP2 (panel A) or PI (panel B) as the substrate. EGFP refers to cells transfected with a control vector expressing EGFP (Clonetech, Palo Alto, CA); MCF-7 refers to untransfected cells; #1, 2, 4, 5, 10, and 11 refer to activities derived from total cell extracts of independent isolates of PLCδ4 overexpressing clones. #2 nuclei refers to activities derived from nuclear extracts of PLCδ4 overexpressing clone #2. Assays were done in triplicates with error bars indicating ± standard deviation (SD). Fusion protein comprised of the PH domain of PLCδ4 and GFP was localized mainly in the cytoplasmic region (panel C).