Figure 4.

Overexpression of PLCδ4 selectively activates PKC-φ in MCF-7 cells. Western blot analysis of MCF-7 cell extracts derived from independent isolates overexpressing PLCδ4 and isolates transfected with control vectors using antibodies against various isoforms of phospho-PKC (left panel) and against the phosphorylated motif of PKC substrates (right panel). Antibodies were from Cell Signaling Technology (Beverly, MA). Blotting was done using the ECL Plus™ detection reagents from Amersham Biosciences (Piscataway, NJ).