Figure 6.

Overexpression of PLCδ4 upregulates ErbB1/2 but does not affect ER-α in MCF-7 cells. Western blot analysis of MCF-7 cell extracts derived from 2 untransfected samples (left 2 lanes), 4 independent isolates overexpressing PLCδ4 and 5 isolates transfected with control vectors (right 5 lanes) using antibodies against Hsp90 (Biosource International, Camarillo, CA), various non-phosphorylated and phosphorylated forms of ER-α and EGFR (Cell Signaling Technology, Beverly, MA), and non-phosphorylated ErbB2 (Upstate Cell Signaling Solutions, Charlottsville, VA). Blotting was done using the ECL Plus™ detection reagents from Amersham Biosciences (Piscataway, NJ).