Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
PLCδ4 overexpressing cells form colonies in soft agar. The cells were plated at 1 × 105 cells/ml in 6-well plates containing RPMI 1640 with 0.35% agar, 5% FBS, 10% tryptose phosphate broth, 100 nM 17-β-estradiol. The picture was taken on day 10 with 40× magnification. Samples were plated on duplicate wells. The two numbers separated by a slash mark shown at the lower right-hand corner of each panel represent the total number of colonies scored in each of the two wells.
