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. 2014 Oct 21;9(10):e109995. doi: 10.1371/journal.pone.0109995

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© 2014 Sawanobori et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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A section of thymus from a Lewis rat was stained with Alexa594-conjugated ED18, Alexa488-conjugated ED21, and Alexa647-conjugated anti-rat MHCII antibody. Pseudocolors were adjusted and assigned with AxioVision software. Merged images are explained schematically below the panels.