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. 2014 Oct 21;9(10):e109995. doi: 10.1371/journal.pone.0109995

Figure 4. Characterization of rat medullary thymic epithelial cells 2: relationship between keratin expression and ED18/ED21.

Figure 4

Sections of thymus from a Lewis rat were stained with Alexa488-conjugated ED18 (A, B) or Alexa488-conjugated ED21 (C). Subsequently, the sections were stained with Alexa647-conjugated anti-rat MHCII antibody, anti-K5 and anti-K8 followed by AMCA-conjugated anti-rabbit IgG and Alexa594-conjugated anti-chicken IgY antibodies, respectively. Pseudocolors were adjusted and assigned with AxioVision software. Pictures in (A) and (B) are from the same multicolor picture with different color assignments. Merged images are explained schematically below the panels.