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. 2014 Oct 21;9(10):e109995. doi: 10.1371/journal.pone.0109995

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© 2014 Sawanobori et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) A section of a thymus from a Lewis rat was stained with Alexa594-conjugated ED18 or ED21, Alexa647-conjugated anti-rat MHCII antibody, and biotin-conjugated UEA-1 followed by Alexa488-conjugated anti-biotin antibody. Pseudocolors were assigned using AxioVision software. (B) The low-density fraction from Lewis rat thymi was subjected to flow cytometric analysis. Cells were stained with Alexa488-conjugated control IgM or ED18/ED21, anti-rat CD45-PE, biotin-conjugated UEA-1 followed by streptavidin-PerCP-Cy5.5, and Alexa647-conjugated anti-rat MHCII.