. 2014 Oct 21;9(10):e109995. doi: 10.1371/journal.pone.0109995

Table 1. Primary antibodies used in this research.

Antigen	Isotype	Clone	Conjugate	Source
unknown	mouse IgM	ED18	−/biotin^#/Alexa488^#/Alexa594^#	a
unknown	mouse IgM	ED19	−/biotin^#/Alexa488^#/Alexa594^#	a
unknown	mouse IgM	ED21	−/biotin^#/Alexa488^#/Alexa594^#	a
keratin 5	rabbit IgG	polyclonal	−	b
keratin 8	chicken IgY	polyclonal	−	c
rat CD45	mouse IgG₁	OX1	−/PE	d/e
rat CD103	mouse IgG₁	OX62	−	f
rat CD205	mouse IgG₁	HD83	−	g
rat MHCII (RT1B^l)	mouse IgG₁	OX3	−/Alexa647^#	h
mouse CD205	rat IgG_2a	NLDC145	−	i
mouse MHCII	rat IgG_2b	M5/114	biotin^#/Alexa647^#	j
autoimmune regulator (AIRE)	goat IgG	polyclonal	−	k
claudin-3	rabbit IgG	polyclonal	−	l
claudin-4	rabbit IgG	polyclonal	−	l
Ulex europaeus Lectin 1 (UEA-1)	−	lectin	biotin/FITC	m
isotype control	mouse IgG	polyclonal	−	n

: conjugated with either biotin conjugation kit (Dojindo) or Alexa conjugation kit (Invitrogen) in house, ^a produced at VU University Medical Center (Amsterdam, Netherland), ^b Assay Biotechnology (Sunnyvale, CA, USA), ^c Abcam (Cambridge, United Kingdom), ^d BD Pharmingen (Franklin Lakes, NJ, USA), ^e Biolegend (San Diego, CA, USA), ^f donated by Dr. M. Brenan, ^g produced at Rockefeller University (NY, USA), ^h ECACC, ⁱ donated by Dr. G. Kraal, ^j eBioscience (San Diego, CA, USA), ^k Sigma-Aldrich (Saint Louis, MO, USA), ^l Invitrogen (Camarillo, CA, USA), ^m Vector Labs (Burlingame, California, CA, USA), ⁿ Jackson Immunoresearch (West Grove, Pennsylvania, USA)