Table 1. Primary antibodies used in this research.
Antigen | Isotype | Clone | Conjugate | Source |
unknown | mouse IgM | ED18 | −/biotin#/Alexa488#/Alexa594# | a |
unknown | mouse IgM | ED19 | −/biotin#/Alexa488#/Alexa594# | a |
unknown | mouse IgM | ED21 | −/biotin#/Alexa488#/Alexa594# | a |
keratin 5 | rabbit IgG | polyclonal | − | b |
keratin 8 | chicken IgY | polyclonal | − | c |
rat CD45 | mouse IgG1 | OX1 | −/PE | d/e |
rat CD103 | mouse IgG1 | OX62 | − | f |
rat CD205 | mouse IgG1 | HD83 | − | g |
rat MHCII (RT1Bl) | mouse IgG1 | OX3 | −/Alexa647# | h |
mouse CD205 | rat IgG2a | NLDC145 | − | i |
mouse MHCII | rat IgG2b | M5/114 | biotin#/Alexa647# | j |
autoimmune regulator (AIRE) | goat IgG | polyclonal | − | k |
claudin-3 | rabbit IgG | polyclonal | − | l |
claudin-4 | rabbit IgG | polyclonal | − | l |
Ulex europaeus Lectin 1 (UEA-1) | − | lectin | biotin/FITC | m |
isotype control | mouse IgG | polyclonal | − | n |
: conjugated with either biotin conjugation kit (Dojindo) or Alexa conjugation kit (Invitrogen) in house, a produced at VU University Medical Center (Amsterdam, Netherland), b Assay Biotechnology (Sunnyvale, CA, USA), c Abcam (Cambridge, United Kingdom), d BD Pharmingen (Franklin Lakes, NJ, USA), e Biolegend (San Diego, CA, USA), f donated by Dr. M. Brenan, g produced at Rockefeller University (NY, USA), h ECACC, i donated by Dr. G. Kraal, j eBioscience (San Diego, CA, USA), k Sigma-Aldrich (Saint Louis, MO, USA), l Invitrogen (Camarillo, CA, USA), m Vector Labs (Burlingame, California, CA, USA), n Jackson Immunoresearch (West Grove, Pennsylvania, USA)