Table 2. Secondary/tertiary antibodies used in this research.
product | conjugate | source |
goat anti-mouse IgM | horse radish peroxidase | MP biomedicals (Santa Ana, California, USA) |
horse anti-mouse IgG | biotin | Vactor Labs |
goat anti-chicken IgY | alkaline phosphatase | Jackson Immunoresearch |
donkey anti-chicken IgY | Alexa594 | Jackson Immunoresearch |
donkey anti-goat IgG | Alexa594 | Jackson Immunoresearch |
donkey anti-rabbit IgG | alkaline phosphatase | Jackson Immunoresearch |
goat anti-rabbit IgG | horse radish peroxidase | MP biomedicals |
donkey anti-rabbit IgG | aminomethyl coumarin acetate (AMCA) | Jackson Immunoresearch |
donkey anti-rat IgG | alkaline phosphatase | Jackson Immunoresearch |
donkey anti-biotin | alkaline phosphatase | Sigma-Aldrich |
goat anti-biotin | Alexa488# | Sigma-Aldrich |
streptavidin | Alexa488, 594 | Invitrogen |
streptavidin | PerCP-Cyanin5.5 | eBioscience |
: conjugated with Alexa conjugation kit (Invitrogen) in house.