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. 2014 Oct 21;9(10):e110603. doi: 10.1371/journal.pone.0110603

Figure 1. Screening and complementation analysis.

Figure 1

Wild type, a ΔpalF isolate, and a ΔpalF isolate that has been transformed with a wild type copy of the palF gene were grown on 3 ml slants of SCM agar medium for 10 days to allow them to form protoperithecia. The three panels show images of the hyphae and protoperithecia on the test tube glass adjacent to the agar slant. An abundance of protoperithecia are produced by the wild type isolate on the glass at the edge of the agar (first panel/A) while the ΔpalF mutant is unable to produce protoperithecia (second panel/B). Transformation of the ΔpalF mutant with a wild type copy of the palF gene restores the ability to generate protoperithecia (third panel/C). Arrows point to examples of protoperithecia.