Figure 2. Perithecia grafting experiments.

Small pieces of cellophane containing “graft” fertilized wild type protoperithecia were placed on mutant “host” vegetative hyphal networks grown on synthetic crossing medium. The “host” shown are: 1) Δfmf-1 (left panel/A) which shows a host supporting the development of the graft perithecia. 2) Δada-1 (middle panel/B) which shows a host not supporting the development of the graft perithecia. 3) ΔpalA (right panel/C) which shows the graft inducing protoperithecia in the ΔpalA host. Arrows point to examples of perithecia on the cellophane (left panel/A) and protoperithecia induced in the host vegetative hyphal network (right panel/C).