Table 3. Examples of clades formed by paleonumts from different orthopteran species.
Numt type | Family | Numt name | Ortholog AT% | NumtAT% | p-distance from ortholog | Age of MRCAbased on fossil data |
COI-like numts | Eumastacidae | OR407_C039 | 0.676 | 0.678 | 0.410 | 145–163.5 MYA [77] |
Pyrgacrididae | OR317_C179 | 0.603 | 0.602 | 0.483 | (Oldest Eumastacidae) | |
COII-like numts | Lentulidae | OR295_C020 | 0.742 | 0.521 | 0.490 | 98.7–108 MYA [75] |
Pamphagidae | OR151_C053 | 0.693 | 0.570 | 0.417 | (Oldest Tridactylidae) | |
Tridactylidae | OR153_C069 | 0.655 | 0.653 | 0.478 | ||
COII-like numts | Lentulidae | OR295_C011 | 0.742 | 0.636 | 0.432 | 33.9–38 MYA [76] |
Pamphagidae | OR151_C069 | 0.693 | 0.629 | 0.386 | (Oldest Acridoidea) | |
ND5-like numts | Lentulidae | OR295_C207 | 0.781 | 0.756 | 0.434 | 182–201 MYA [74] |
Pamphagodidae | OR540_C135 | 0.725 | 0.728 | 0.521 | (Oldest Prophalangopsidae) | |
Pamphagodidae | OR540_C121 | 0.725 | 0.516 | 0.505 | ||
Pneumoridae | OR293_C021 | 0.757 | 0.674 | 0.440 | ||
Prophalangopsidae | OR021_C021 | 0.709 | 0.584 | 0.416 | ||
Prophalangopsidae | OR021_C022 | 0.709 | 0.565 | 0.402 |
The recovery of these clades indicates that the nuclear insertion event probably occurred in the most recent common ancestor (MRCA) of the species forming the clades. Numt name indicates the specific cloned sequence number used in the study, available in Table S3. Ortholog AT% indicates the base composition of the orthologous mtDNA sequence of the corresponding numt. Numt AT% is the base composition of the specific numts below to show how similar or different they are from the ortholog. p-distance from ortholog indicates the uncorrected p-distance of the numt sequence from the corresponding ortholog. In general, these paleonumts are highly divergent from the orthologs. Age of MRCA based on fossil data is determined from the oldest known fossil for particular clades, thus showing the maximum date of nuclear insertion.