Figure 6.

TTR species analyzed by semi-native PAGE (nonboiled samples) and visualized consecutively by resveratrol fluorescence staining (left panel) and Coomassie staining (right panel). The samples analyzed are WT TTR, FT₂·WT TTR, an equimolar mixture of WT TTR and FT₂·WT TTR that had previously been unfolded and refolded to allow the formation of mixed heterotetramers (labeled WT/FT₂·WT), and a well characterized engineered monomeric TTR variant, M-TTR.²⁰ When resveratrol is bound to tetrameric TTR species, it is fluorescent, demonstrating that semi-native PAGE conserves TTR tetrameric quaternary structure.