. 2014 Sep 23;53(41):6496–6510. doi: 10.1021/bi500739q

Table 1. Midpoint Transition Values (C_m) of TTR Denaturation–Renaturation Curves in Urea.

	refolding		tetramer reassembly		unfolding		tetramer disassembly
	C_m^a	p^b	C_m	p	C_m	p	C_m	p
WT	3.5 ± 0.01		3.3 ± 0.03		3.6 ± 0.04		3.2 ± 0.04
FT₂·WT	3.3 ± 0.01	**	3.1 ± 0.03	*	3.4 ± 0.03	*	3.0 ± 0.05	ns
V122I	3.4 ± 0.02		3.0 ± 0.03		3.5 ± 0.03		2.9 ± 0.04
FT₂·V122I	3.2 ± 0.02	**	2.6 ± 0.03	**	3.2 ± 0.02	**	2.5 ± 0.03	**

C_m values in molar (M) units. TTR unfolding and refolding were measured by tryptophan fluorescence; tetramer disassembly and reassembly were measured by resveratrol-binding fluorescence.

p values measure the significant changes in C_m for the flag-tagged proteins with respect to their non-flag-tagged counterparts. Unpaired Student’s t test: ns (not significant), p > 0.05, * p ≤ 0.05, ** p ≤ 0.01.

Table 1. Midpoint Transition Values (Cm) of TTR Denaturation–Renaturation Curves in Urea.

Table 1. Midpoint Transition Values (C_m) of TTR Denaturation–Renaturation Curves in Urea.