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Images generated of m/z 104.1 (choline), m/z 132.1 (leucine), m/z 147.1 (glutamine), m/z 269.1 (unknown), and m/z 616.2 (heme, [M+]) by applying DHB with the airbrush (top), optimized automatic sprayer method (middle), and optimized sublimation method (bottom) to serial sections of Medicago truncatula root nodules.
