Archival storage of scWestern slides with subsequent
successful antibody probing. (A) Optical profilometer images of hydrated
and dehydrated scWestern PA gel features. Circular
features are microwells, 30 μm in diameter. (B) False-color
fluorescence micrographs and intensity traces of the same scWestern device showing antibody probing for GAPDH (blue
signal) after fresh preparation and after 4 months of archival storage.
(C) Fluorescence micrographs of the same scWestern
device reprobed for β-tubulin (blue signal). AU, arbitrary unit. E = 40 V cm–1, lysis time = 25 s, electrophoresis
time = 20 s, 12%T PA gel (GAPDH, Alexa Fluor 647-labeled secondary
antibody; β-tubulin, Alexa Fluor 594-labeled secondary antibody).