Table 2. Thermodynamic Data from DSC Analysis.
| lipid | vesicle | Tm (°C)a | ΔH (kcal/mol) |
|---|---|---|---|
| DPPC | LUV | 41.1 ± 0.2 | 8.8 ± 1.0 |
| MLV | 41.2 ± 0.1 | 8.7b | |
| DPPC/Chol 95:5 | LUV | 40.1 ± 0.2 | 5.3 ± 0.9 |
| MLV | 40.4 ± 0.1 | ||
| 90:10 | LUV | 40.4 ± 0.4 | 6.3 ± 1.0 |
| MLV | 40.4 ± 0.1 | ||
| 80:20 | LUV | 41.3 ± 0.4 | 3.0 ± 0.5 |
| MLV | 41.2 ± 0.1 | ||
| DPPC/DChol 95:5 | LUV | 40.1 ± 0.5 | 7.7 ± 0.2 |
| MLV | 40.4 ± 0.1 | ||
| 90:10 | LUV | 40.3 ± 0.5 | 7.5 ± 1.6 |
| MLV | 40.1 ± 0.4 | ||
| 80:20 | LUV | 40.8 ± 0.1 | 4.6 ± 0.3 |
| MLV | 40.6 ± 0.2 |
a
Tm is defined as the position of the maximum in the heat capacity curve. The values shown correspond to means and standard deviations (SD) of more than 20 determinations in DPPC and DPPC/Chol mixtures (except for DPPC/Chol (95:5), which had only 4 determinations) and 2 to 3 determinations for DPPC/DChol. These SD values do not explicitly take into account the errors associated with baseline corrections and phosphate analyses needed to calculate ΔH. We estimate that the inclusion of those sources of error results in a relative error of 10–15% in ΔH.