Figure 6.

(a) Process of single-CTC isolation using a LCM system includes (I) CTC identification; (II) determination of IR sticky finger positions and UV dissection route; (III) UV laser dissection; (IV) collection of the identified CTC onto the LCM cap. (b) Circos plots representing the coverage areas of Exome-Seq. The rings from the inside out represent WBCs, pooled CTCs (CTCp), CTC-1, and CTC-2. The outermost ring represents the karyotype of the human reference genome. (c) The shared mutations between CTCs and WBC are compared with the shared mutations among CTCs. Adapted with permission from ref (29). Copyright 2013 John Wiley and Sons.