TABLE 1.
Primary antibodies tested on leukocytes and other cell types.
| Antibody | Manufacturer (cat. no.) | Used conc. | Cell | Main localization(s) | Target species | Refs. |
|---|---|---|---|---|---|---|
| Aph-1 (γ secretase complex subunit) | Abcam (ab24613, rabbit IgG) | 5 μg/ml | Eosinophils | Golgi, secretory granules and peripheral cytoplasm | Human | 22 |
| CD9 | R&D (MAB1880, clone 209306, mouse IgG2B) | 10–20 μg/ml | Eosinophils | Cell surface | Human | 24 |
| CD63 | BD-Pharmigen (556019, clone H5C6, mouse IgG1) | 2 μg/ml | Eosinophils | Secretory granules (limiting membrane and intragranular pool) | Human | 17,23 |
| IL-4 | R&D (MAB604, clone 3010, mouse IgG) | 2 μg/ml | Eosinophils | Transport vesicles and granules | Human | 20 |
| IL-4Rα | Santa Cruz Biotechnology (sc-685, rabbit IgG) | 1 μg/ml | Eosinophils | Transport vesicles, granules and peripheral cytoplasm | Human | 21 |
| LC3A | Sigma-Aldrich (L8793, rabbit IgG) | 0.5 μg/ml | Macrophages and cardiomyocytes | Autophagosomes | Mouse | a |
| MBP | BD-Pharmingen (554253, clone AHE2, mouse IgG1) | 5 μg/ml | Eosinophils | Transport vesicles and secretory granules | Human | 17 |
| Nitric oxide synthase 3 (NOS3) | Santa Cruz Biotechnology (s-654, rabbit IgG) | 10 μg/ml | Platelets | α-Granules surface | Mouse | a |
| Notch 1 (anti-activated form) | Abcam (ab8925, rabbit IgG) | 5 μg/ml | Eosinophils | Secretory granules and plasma membrane | Human | 22 |
| Notch 1 (anti-activated form, cleaved-Val1744) | Abcam (ab52301, rabbit IgG) | 10 μg/ml | Eosinophils | Secretory granules, vesicles and cytoplasm | Human | a |
| Notch ligand (Jagged 1) | Santa Cruz Biotechnology (sc-8303, rabbit IgG) | 5 μg/ml | Eosinophils | Plasma membrane and secretory granules | Human | 22 |
| Notch ligand (Jagged 2) | Santa Cruz Biotechnology (sc-5604, rabbit IgG) | 5 μg/ml | Eosinophils | Plasma membrane and secretory granules | Human | 22 |
| Perilipin-2/ADRP | Fitzgerald (20R-AP002, guinea pig IgG) | 2 μg/ml | Macrophages | Lipid bodies | Human/mouse | 34 |
| PDI | Abcam (ab2792, clone RL90, mouse IgG2a) | 5 μg/ml | Eosinophils | Nuclear envelope, granules, vesicles and lipid bodies | Human | 27 |
| Rab7 | Santa Cruz Biotechnology (sc-10767, rabbit IgG) | 10 μg/ml | Macrophages, neutrophils | Phagosomes | Mouse | a |
| Syntaxin 17 | Santa Cruz Biotechnology (sc-1070095, goat IgG) | 5 μg/ml | Eosinophils | Granules and vesicles | Human | a |
a
Unpublished observations.