Table 1. Evolutionary analysis of TRPV1-4 channels for directional positive selection based on multiple sequence alignments.
| Vertebrates | ||||
| TRPV1 | TRPV2 | TRPV3 | TRPV4 | |
| substitution/site | 4.49 | 8.57 | 2.43 | 3.21 |
| residues in alignment | 964 | 915 | 829 | 906 |
| sequences | 25 | 28 | 21 | 22 |
| sites under positive selection* | 100 | 158 | 69 | 177 |
| Mammals | ||||
| TRPV1 | TRPV2 | TRPV3 | TRPV4 | |
| substitution/site | 1.88 | 3.66 | 1.19 | 0.33 |
| residues in alignment | 875 | 821 | 811 | 872 |
| sequences | 20 | 22 | 18 | 11 |
| sites under positive selection* | 72 | 106 | 37 | 13 |
*considering as significant the top 5% sites in the FADE analysis (0.95 posterior filter).