Table 2.
Evaluation of various culture media and incubation conditions for enumerating pure cultures of bifidobacteria and lactic acid bacteria strains*.
| # | Bacterial species and strain | Culture medium†, incubation temperature, incubation time, and atmospheric conditions | |||||||
|---|---|---|---|---|---|---|---|---|---|
|
|
|||||||||
| CASO agar‡ | TOS-MUP agar, 37 °C, 72 h, anaerobic | MRS-CC agar, 37 °C, 72 h, anaerobic | MRS pH 6.2 agar, 37 °C, 72 h, anaerobic | MRS pH 5.4 agar, 37 °C, 72 h, anaerobic | MRS pH 5.4 agar, 45 °C, 48 h, anaerobic | M17 agar, 37 °C, 48 h, aerobic | M17 agar, 45 °C, 24 h, aerobic | ||
| 1 | Bifidobacterium animalis subsp. lactis BB-12 | 8.33 ± 0.18b | 9.30 ± 0.12a | < 6.00 | 8.07 ± 0.14b | < 6.00 | < 6.00 | < 6.00 | < 6.00 |
| 2 | Bifidobacterium breve M-16 V | 8.05 ± 0.11c | 8.76 ± 0.02a | < 6.00 | 8.61 ± 0.04b | < 6.00 | < 6.00 | < 6.00 | < 6.00 |
| 3 | Lactobacillus acidophilus LA-5 | 8.20 ± 0.05b | < 6.00 | 8.30 ± 0.10ab | 8.34 ± 0.02a | 8.33 ± 0.02a | 8.42 ± 0.11a | 7.84 ± 0.07c | < 6.00 |
| 4 | Lactobacillus acidophilus NCAIM B.02085 | 8.22 ± 0.06b | < 6.00 | 8.54 ± 0.17a | 8.54 ± 0.04a | 8.54 ± 0.03a | 8.51 ± 0.14a | 8.17 ± 0.09b | < 6.00 |
| 5 | Lactobacillus casei NCAIM B.01137 | 7.70 ± 0.19a | < 6.00 | 7.30 ± 0.24a | 7.58 ± 0.08a | 7.56 ± 0.08a | < 6.00 | 7.76 ± 0.28a | 7.45 ± 0.17a |
| 6 | Lactobacillus casei HDRI-R | 7.86 ± 0.15a | < 6.00 | 7.88 ± 0.18a | 7.80 ± 0.17a | 7.64 ± 0.12a | < 6.00 | 7.20 ± 0.15b | 7.30 ± 0.11b |
| 7 | Lactobacillus delbrueckii subsp. bulgaricus YC-X11 | 6.30 ± 0.18b | < 6.00 | < 6.00 | 6.78 ± 0.05a | 6.48 ± 0.09b | < 6.00 | < 6.00 | < 6.00 |
| 8 | Lactobacillus delbrueckii subsp. bulgaricus CH-2 | 6.70 ± 0.18b | < 6.00 | < 6.00 | 7.11 ± 0.09a | 6.30 ± 0.33b | < 6.00 | < 6.00 | < 6.00 |
| 9 | Streptococcus thermophilus TH-4 | 7.43 ± 0.12a | < 6.00 | < 6.00 | < 6.00 | < 6.00 | < 6.00 | 7.57 ± 0.13a | 6.78 ± 0.18b |
| 10 | Streptococcus thermophilus DSM 20479 | 8.05 ± 0.22a | < 6.00 | < 6.00 | < 6.00 | < 6.00 | < 6.00 | 7.89 ± 0.17a | 6.90 ± 0.18b |
| 11 | Lactococcus lactis subsp. lactis ATCC 19435 | 7.98 ± 0.17a | < 6.00 | < 6.00 | 7.81 ± 0.07a | < 6.00 | < 6.00 | 7.71 ± 0.19a | < 6.00 |
| 12 | Lactococcus lactis subsp. lactis biovar. diacetylactis VK-256 | 7.18 ± 0.12a | < 6.00 | < 6.00 | < 6.00 | < 6.00 | < 6.00 | 6.30 ± 0.27b | < 6.00 |
| 13 | Leuconostoc mesenteroides subsp. dextranicum ATCC 19255 | 7.80 ± 0.18a | < 6.00 | < 6.00 | < 6.00 | < 6.00 | < 6.00 | < 6.00 | < 6.00 |
*
Values are log10 cfu/mL means ± SD based on four observations (two samples, two replicates).
abc
Means within a row without a common superscript differ (p < 0.05).
†
For composition and/or preparation of culture media, see subsection “Culture media”.
‡
Incubated at 37 °C for 72 h anaerobically (strain #1 to #8), at 37 °C for 48 h aerobically (strain #9 and #10), or at 30 °C for 72 h under aerobic conditions (strain #11 to #13).