Table 1.
Bacterial strains and plasmids used in this study.
| Strain | Plasmid | Description | Reference or source |
|---|---|---|---|
| P. fluorescens 07A | Wild type | (Martins et al., 2005) | |
| P. fluorescens 07A-2 | pMLBAD-aiiA-Trmr-Gmr | Transconjugant, express the lactonase enzyme | This study |
| P. fluorescens 041 | Wild type | (Martins et al., 2005) | |
| P. fluorescens 041-3 | pMLBAD-aiiA-Trmr-Gmr | Transconjugant, express the lactonase enzyme | This study |
| E. coli MT102 | pSB403, Tcr | Monitor strain: exhibits the highest sensitivity for 3-oxo-C6-HSL. However, several other AHL molecules are detected by this sensor. | (Winson et al., 1998; Viana, 2006) |
| E. coli XL1-Blue | pMLBAD-aiiA- Trmr | Donor of pMLBAD-aiiA, Trmr that codify the lactonase enzyme | (Wopperer et al., 2006) |
| E. coli XL1-Blue | pMLBAD-aiiA- Trmr-Gmr | Donor of pMLBAD-aiiA, Gmr that codify the lactonase enzyme | This study |
| E. coli HB101 | pRK600, Cmr | Helper | Laboratory of Microbiology, University of Zürich |