Table 1.
Adherence to and invasion of HEp-2 cells by Edwarsiella tarda studied with light microscopy and acridine orange-crystal violet staining and controlled by scanning electronic microscopy (SEM) and transmission electronic microscopy (TEM), respectively.
| Strains | Adhesion percentage (%) | N° of adhered bacteria/cell ± SD | SEM adherence control | Invasion percentage (%) | N° of invading bacteria/cell ± SD | TEM invasion control |
|---|---|---|---|---|---|---|
| 1 | 70 | 2.88 ± 2.46 | + | 74 | 1.68 ± 1.30 | + |
| 3 | 56 | 3.76 ± 1.76 | + | 53 | 1.32 ± 1.07 | + |
| 4 | 86 | 6.6 ± 2.59 | ND | 58 | 2.92 ± 1.00 | ND |
| 7 | 92 | 5.38 ± 2.89 | ND | 47 | 2.76 ± 0.93 | ND |
| 8 | 78 | 5.26 ± 3.76 | ND | 54 | 2.24 ± 1.22 | ND |
| 9 | 93 | 12.1 ± 7.66 | ND | 37 | 3.62 ± 1.22 | ND |
| 10 | 98 | 17.22 ± 6.36 | + | 46 | 5.32 ± 1.71 | + |
| 11 | 96 | 9.4 ± 6.48 | + | 42 | 3.28 ± 1.75 | + |
| 15 | 94 | 8.22 ± 6.19 | ND | 48 | 3.94 ± 1.07 | ND |
| 17 | 100 | 9.52 ± 4.01 | + | 39 | 4.12 ± 1.72 | + |
| 20 | 99 | 13.66 ± 7.01 | ND | 30 | 4.42 ± 0.70 | ND |
| 22A | 90 | 5.96 ± 3.12 | ND | 21 | 2.30 ± 0.81 | ND |
| 22B | 93 | 14.62 ± 8.30 | + | 39 | 4.74 ± 1.19 | + |
| 23 | 98 | 20.5 ± 8.05 | + | 50 | 5.84 ± 1.33 | + |
| 25 | 96 | 15.5 ± 9.01 | + | 61 | 4.56 ± 1.38 | + |
| 26 | 94 | 8.08 ± 4.26 | ND | 59 | 2.12 ± 1.08 | ND |
| 27 | 90 | 7.84 ± 4.46 | + | 51 | 2.88 ± 1.11 | + |
SD = standard deviation; SEM = scanning electron microscopy; ND = not done.
TEM = transmission electron microscopy.