Table 2.
Summary of computational analysis a of the transcripts obtained from C. gattii grown in low iron medium for 12 h at 37 °C.
| Annotation | E valueb | Access number (Genbank)c | Frequency (number of clones)d | Fold-change |
|---|---|---|---|---|
| Ergosterol metabolism | ||||
| C-4 methyl sterol oxidase | 2E-116 | XP_569528.1 | 194 | 2.02 |
| Protein catabolism | ||||
| ubiquitin-protein ligase | 2E-37 | XP_572505.1 | 11 | 1.40 |
| Cellular respiration | ||||
| mitochondrion protein | 7E-22 | XP_571658.1 | 13 | 1.30 |
| 5-aminolevulinate synthase | 7E-106 | XP_569249.1 | 09 | 0.52 |
| Lipid metabolism | ||||
| Delta-12 fatty acid desaturase | 2E-37 | XP_570226.1 | 01 | 0.65 |
| Transporters | ||||
| ABC transporter | 5E-28 | XP_568169.1 | 02 | 2.42 |
| inorganic phosphate transporter | 1E-90 | XP_568544.1 | 03 | 1.20 |
| mRNA export factor elf1 | 9E-66 | XP_566522.1 | 01 | 1.90 |
| Cell wall or membrane organization | ||||
| diacylglycerol cholinephosphotransferase | 1E-71 | XP_571616.1 | 21 | 2.60 |
| Stress response | ||||
| Heat shock protein 90 | 7E-39 | XM_568451.1 | 01 | 2.10 |
| Rds1 protein | 1E-05 | XP_568514.1 | 08 | 5.70 |
| Translation | ||||
| polyadenylate-binding protein | 4E-22 | XP_572834. 1 | 04 | 1.35 |
| ribosomal protein L1 | 3E-48 | XP_569608.1 | 01 | 0.85 |
| Carbohydrate metabolism | ||||
| Glucosidase | 2E-24 | XP_570389.1 | 02 | 1.90 |
| Cell cycle | ||||
| g1/s-specific cyclin pcl1 (cyclin hcs26) | 7E-29 | XP_571897.1 | 01 | 0.60 |
| alpha tubulin | 2E-57 | XP_568869.1 | 02 | 1.20 |
| Nucleotide metabolism | ||||
| guanosine-diphosphatase | 2E-86 | XP_568971.1 | 02 | 0.75 |
| Others | ||||
| minor histocompatibility antigen h13 | 5E-34 | XP_572261.1 | 01 | 0.80 |
| nucleus protein | 1E-28 | XP_567927.1 | 11 | 1.0 |
| Hypothetical proteins | ||||
| hypothetical protein | 2E-24 | XP_571639.1 | 7 | 0.71 |
| hypothetical protein CNBM2310 | 1E-60 | XP_772074.1 | 80 | 2.44 |
| hypothetical protein CNBH2900 | 3E-42 | XP_773838.1 | 02 | 0.87 |
| hypothetical protein CNBC2910 | 2E-23 | XP_776800.1 | 01 | 0.30 |
| hypothetical protein | 2E-36 | XP_570947.1 | 01 | - |
| hypothetical protein CNBC4100 | 6E-23 | XP_776919.1 | 01 | 1.85 |
| hypothetical protein | 4E-18 | XP_570177. 1 | 01 | 1.50 |
| hypothetical protein CNBM0150 | 4E-40 | XP_772247.1 | 04 | - |
| hypothetical protein CNM02470 | 5E-108 | XP_568428.1 | 45 | - |
| hypothetical protein CNBM0150 | 9E-33 | XP_772247.1 | 12 | - |
| Control Iron genes e | ||||
| high-affinity iron permease CaFTR1 | AFR98470.1 | 22.15 | ||
| Multicopper oxidase | AFR98469.1 | 11.77 | ||
| Ion regulator 1 | AFR97352.1 | 0.50 | ||
| bZIP transcription factor | AFR94904.1 | 8.08 | ||
a
Sequence quality was analyzed using the Phred Program, and contig assembly was performed by using the Staden Package Software. Resulting sequences were compared to the Genbank database using the BLASTX program.
b
E value according to information from BLASTX searches of the non-redundant database at NCBI.
c
Accession number of the gene products in the GenBank database.
d
Frequency represents the number of clones found in a total of 442 high quality sequenced cDNA clones.
e
Genes used to validate iron deprivation conditions represented by reductive, high affinity iron transport and transcriptional factors.