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. 2014 Oct 21;9(10):e110920. doi: 10.1371/journal.pone.0110920

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© 2014 Pignatelli et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) H&E staining of isolated leukocyte populations from the rainbow trout AT. Scale bar, 5 µm. (B) Flow cytometry analysis of leukocytes isolated from the AT stained with MAbs against trout IgM, IgT, IgD, CD8 and MHC-II. Dot plots show a FSC/SSC profile of isolated AT-resident leukocytes including the leukocyte gate and corresponding antibody fluorescence intensity versus FSC of gated leukocytes. (C) Mean percentage of IgM⁺, IgT⁺, IgD⁺, CD8α⁺ and MHC-II⁺ leukocytes in the gated population (n = 8).