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. 2014 Jul 25;9(10):2318–2325. doi: 10.1021/cb500230b

Figure 2.

Figure 2

TD-1 increases the oxygen affinity of normal human hemoglobin (Hb) and whole blood. (A) The ODC of hemoglobin (Hb, 20 μM as tetramer) without and with TD-1 (120 μM in DPBS, pH 7.4, with 5 vol% DMSO) at 37 °C. (B) The P50 of hemoglobin mixed without and with Compound 1 (120 μM) or TD-1 (20, 40, 80, and 120 μM). (C) Relationship between pH and P50 of hemoglobin (20 μM) with and without addition of TD-1 (120 μM) in 0.1 M phosphate buffer (pH 6-8) with 5 vol% DMSO at 37 °C. (D) The ODC of whole blood without or with TD-1 (40 μM, 2:1 molar ratio to Hb tetramer) in Hemox solution with 0.8 vol% DMSO at 37 °C. (E) The P50 of whole blood mixed with TD-1 (20, 40, 80, and 120 μM). Each symbol represents the mean value of P50 measured in triplicate. (F) The P50 of whole blood mixed with TD-1 (40 μM), 5-HMF, formamidine disulfide, and NEM (all 120 μM). Each bar represents the mean value of P50 measured in triplicate. Error bars represent standard deviation.