Table 2. Mass spectrometry analysis of phage ArV2 proteins.
Gene | Putative Function | MW (KDa) | Peptide count | Sequence coverage (%) |
ORF15* | tape measure protein | 127.953 | 74 | 35.2 |
ORF24* | tail fiber protein | 52.293 | 11 | 38.0 |
ORF03* | phage portal protein | 50.447 | 60 | 79.3 |
ORF17* | tail protein | 40.386 | 42 | 70.1 |
ORF16* | tail protein | 35.644 | 26 | 58.3 |
ORF18* | tail protein | 33.604 | 39 | 84.9 |
ORF04f | capsid maturation protease | 31.057 | 19 | 63.3 |
ORF06* | major capsid protein | 30.937 | 21 | 78.9 |
ORF11* | major tail protein | 23.801 | 72 | 95.9 |
ORF23u | hypothetical protein | 20.621 | 16 | 95.4 |
ORF07 | hypothetical protein | 14.566 | 13 | 91.2 |
ORF10 | hypothetical protein | 14.563 | 9 | 70.4 |
ORF08 | hypothetical protein | 12.709 | 7 | 78.8 |
ORF09* f, u | hypothetical protein | 10.230 | 10 | 76.2 |
* – putative structural ArV2 proteins identified by bioinformatics approaches;
f – proteins detected using the FASP method only;
u – ArV2-specific proteins that have no reliable identity to database entries.
Proteins were identified as a result of whole-phage shotgun analysis (WSA) using LC MS/MS analysis and Mascot search against an in-house database.