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. 2004 Jun 15;113(12):1774–1783. doi: 10.1172/JCI20513

Figure 4.

Figure 4

Expression of adipogenic and lipogenic genes in the absence of hepatic Pten. (A) RT-PCR analyses of the indicated genes in +/+, flox/+, and flox/flox livers. The relative intensity of each amplified PCR band as measured by NIH Image was as follows: PPARγ +/+, 1; flox/+, 2; flox/flox, 8; PPARα (1, 1, 1); C/EBPα (1, 1, 1); C/EBPβ (1, 1, 0.7); C/EBPδ (1, 1, 1); SREBP1c (1, 1.5, 3); adiponectin (1, 1, 4); adipsin (1, 1, 4); aP2 (1, 1, 4); FAS (1, 1, 3); ACC (1, 1, 2); SCD1 (1, 1, 2); AOX (1, 1, 2); L-PBE (1, 1, 2); PTL (1, 1, 2.5); β-actin (1, 1, 1). Data shown are representative of five independent experiments. (B) Expression of adipocyte-specific and hepatocyte-specific markers in the liver. Immunohistochemical double staining using antibodies specific for adiponectin and albumin revealed the strong coexpression of these molecules in flox/flox liver cells (lower panel) compared with +/+ liver cells (upper panel). Magnification ∞1000.