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. 2004 Jun 15;113(12):1711–1721. doi: 10.1172/JCI20926

Figure 2.

Figure 2

Ung–/– MEFs are susceptible to nitric oxide toxicity. (A) Ung–/– and Ung+/+ MEF were subjected to different doses of SNP for 24 hours, which is known to induce deaminative damage. After 48 hours, viable cells were counted by trypan blue staining (mean ± SE of determinations made in three different experiments; *P < 0.05 and **P < 0.01 compared with the values in Ung+/+ MEF exposed to SNP; ANOVA followed by Scheffe’s post hoc test). (B) Increased uracil content in DNA in Ung–/– MEF after exposure to SNP. DNA was prepared from Ung–/– and Ung+/+ MEF subjected to different doses of SNP, enzymatically hydrolyzed to free nucleosides, and subjected to reverse-phase HPLC. Ratios of deoxyuridine (dUridine) to deoxycytidine (dCytidine) were determined. Values are mean ± SE from three independent experiments measured in duplicate; ANOVA followed by Scheffe’s post hoc test. ***P < 0.005.